Cellulase Enzyme

Product Introduction:
Americos K255 is a concentrated powder cellulase enzyme which can effectively be used for bio-polishing and denim abrasion of cellulase composed garments. It is a cellulose enzyme preparation produced using genetically modified strain of Trichoderma reesei. The enzyme is designed to be efficient in treating of cellulosic material and is especially suitable for the finishing processes of all types of cellulosic fabrics.

Product Application:
The product is applied in a novel enzymatic finishing process, for cellulose based fabrics, which is known as “Bio-Polishing” as well as creating denim abrasion also known as stone washing effect.

Application Parameters:

pH	5.5-7.0
Temp	40°C-65°C
Dosage	0.01%-0.07%

Final conditions will vary according to fabric type, conditions, as well as customer style preference.

Dosage Recommendations:
It is recommended that optimal dosage be defined by experimenting to obtain the desired effects. Customer’s desired affects varies by company so our listed dosage should be used as reference only.

Compatibility:
Non Ionic wetting agents, non-ionic surfactants, dispersants, buffering salts, and pumice stone is compatible with Americos K255, but positive testing is recommended prior to all formulations and applications. Contact of Americos K255 with ferric ions should be avoided.

Inactivation:
Inactivation must be carried out after treatment. Either alkaline rinse by raising pH to > 10 for 10 minutes or the use of bleaching agents will terminate the enzyme reaction.

Storage & Handling Precautions:

• Store in cool place and avoid storing in direct sunlight
• The shelf life of Americos K255 is one years under recommended storage
• The usual precautions in keeping chemicals away from eyes and skin should be observed

Technical Service :
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.

Product Introduction:
Americos K 27 is a highly concentrated acid cellulose enzyme 5000 ECU/g produced by genetically modified strains of Aspertillus oryzae. It is cost effective for denim / knitted material.

Americos K 27 produced by submerged fermentation of genetically modified micro organism. The enzyme protein, which is protein engineered, is separated and purified from the production organism.
Advantages

• Better and bigger granular effect
• Maximized fabric strength retention
• High color contrast finish
• Optimum strength retention
• Easy handling
• High degree of reproducibility and reliability
• Cost-effectiveness
• Improved wash look or creation of new looks
• Low degree of indigo back-staining
• Even abrasion
• Improves touch and drape of garments
• High contrast finish

General properties

Appearance	Powder
Odor	Slightly fermented odor
Chemical character	Aspertillus oryzae
Physical form	Granulated
Solubility	Readily soluble in water

Application Methods
Americos K 27 is applied on denim garment using rotary drum washer with high mechanical action.

Recipe

Americos K 27	0.5 - 1% (owg)
Anti-crease pro 76 A	0.5 - 1 g/l
Americos White MRC	1- 4% (owg)
Temperature	50° – 70°C
pH	5.5 - 6.5
Time	45–90 min

Stability

Hard water	Good
Acids/Alkalies	Active and stable in pH range 4.5 - 8.5
Metal ions	Poor, metal ions are poisonous for cellulase

Compatibility

Non-ionic surfactant	Good
Anionic surfactant	Selective, some reduce the efficacy of the enzyme
Solvent based surfactant	Selective, some reduce the efficacy of the enzyme
Organic sequestrants	Generally good
Reducing / oxidizing agent	Reduce the efficacy of enzyme

Storage & Handling Precautions

• Store in cool place
• Avoid storing in direct sunlight
• The shelf-life of Americos K 27 is one year if stored below 40°C
• The usual precautions for keeping chemicals away from eyes and skin should be observed

Technical Service
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.

Product Introduction:
Americos K 72 is a concentrated base product intended for incorporation in stone washing formulations as a base ingredient.


Characteristics:

Physical form	Formulated granulate
Activity	4500 ECU/g
Appearance	Off white
Odor	Slightly fermentation
Enzyme organism	Aspergillus oryzae

Benefits:
Americos K 72 is a high-performance product that allows new shades and finishes to be created easily and cost-effectively in an environmentally friendly way.

Americos K 72 is recommended when the following are sought:

• Optimum strength retention
• Low degree of indigo back-staining
• High color contrast finish
• Improved wash look or creation of new looks
• Maximised fabric strength retention
• High degree of reproducibility and reliability

Performance:
The performance of Americos K 72 depends on temperature and pH as shown in

Influence of temperature on the performance of Americos K 72


Influence of pH on the performance of Americos K 72

Application/process:
Americos K 72 is a highly concentrated base product for the abrasion of denim (stone washing). It is not suitable for direct use due to its high activity but should be incorporated into stone washing formulations as a base ingredient

With stones:

Americos K 72	1-3% o.w.o.g.
Liquor ratio	4:1-15:1 l/kg
Time	40-60 min
pH	6.5-7.5
Temperature	45-60°C (113-140°F) - (target 55°C)
Stones	0.5-2 kg/kg garment

Without stones:

Americos K 72	1-3% o.w.o.g.
Liquor ratio	4:1-15:1 l/kg
Time	60-90 min
pH	6.5-7.5
Temperature	45-60°C (113-140°F) - (target 55°C)
Stones	0.5-2 kg/kg garment

The enzyme treatment should be completed with a thorough rinse and detergent wash-off. inactivation of enzyme, run machine for 15 minutes with 1-2 g/l sodium carbonate (pH ³ 10) at 80°C (176°F). For optimum clean-up, add 0.2-1 g/l of a dispersing agent Americos Superior Basta.

Storage & Handling Precautions

• Store in a cool place
• Avoid storing in direct sunlight
• The shelf life of Americos K 72 is one year if stored below 25°C
• The usual precaution for chemicals should be observed

Technical Service
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.

Introduction:
Americos K - 920 is a bio-polishing enzyme. The term bio-polishing refers to the removal of fluffy fibers / protruding fibers from the surface of fabric by enzymatic hydrolysis. Such bio-polishing results in clear fabric surface. Americos K - 920 has three different enzyme activities: Exo-Cellulase, Endo-Cellulase and ß-Glucosidase which makes Americos K - 920 especially suitable for all kinds of cellulosic fibers.

Americos K - 920 enzymatically hydrolyses the protruding fibers and loosens their ends. The removal of such hydrolyzed fibers requires mechanical agitation. Hence, the machines like soft-flow, jet and wash wheel, which produce mechanical agitation, are more suitable for bio-polishing.

Advantages:

• Clear fabric surface
• Reduced pilling
• Improved softness and smoothens
• Improved drape

Properties:

Appearance	Liquid
Odor	Slightly fermented odor
Chemical character	Cellulase (Endo,1-4-8 D-Glucanase)
Solubility	Readily soluble in water
pH (1% solution)	7 ± 1
pH Application	4.5 – 5.5
Application temperature range	60°C

Stability:

Hard water	Good
Acids/ Alkalies	Active and stable form in pH range 4.5–8.5
Metal ions	Metal ions are poisonous for cellulase

Compatibility:

Non-ionic surfactants	Good
Anionic surfactants	Selective, some reduce the efficacy of the enzyme
Solvent based surfactants	Selective, some reduce the efficacy of the enzyme
Organic sequestrants	Generally good
Reducing/ oxidizing agents	Reduce the efficacy of enzyme

Application:
Americos K - 920 is applied on fabric / garment in soft flow, rotary drum washer with high mechanical action.

Dosage:

Americos K - 920	0.75 – 1% (owg)
Americos Anti-Crease Pro 76A	0.5 – 1 g/l
Temperature	55 – 60°C
pH	4.5 – 5.5
Time	30 – 90 min

Storage & Handling Precautions:

• Americos K - 920 should be stored below or at ambient temperature.
• Avoid storing in direct sunlight.
• The shelf-life of Americos K - 920 is one year if stored below 40°C.
• The usual precaution in keeping chemicals away from eyes and skin should be observed.

Technical Service:
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.