Introduction :
Americos Amylase 180 is a desizing agent based on bacterial alpha amylase. It is a unique blend of thermal stable alpha-amylase and lubricant to remove most of the size materials from denim garment. This unique combination prevents the formation of unwanted creases, streaks, lines and crack marks during desizing process. It can be used to degrade the following size materials and their combinations:

• Starch
• Starch / Polyvinyl alcohol
• Starch / Polyvinyl alcohol / Carboxyl Methyl cellulase (CMC)
• Starch / Polyvinyl alcohol / Acrylates

Properties :

Appearance	Liquid
Chemical character	Bacterial amylase enzyme stabilized with buffer
Ionicity	Nonionic
Solubility	Readily soluble in water
pH (on 1% solution)	7 ± 1
Application pH range	5 - 8
Application temperature range	60oC

Stability :

Hard water	Good. Ca2+ and Mg2+ ions increase the efficacy of the enzyme
Acids	Good to pH 5 to pH 7
Alkalis	Stable in alkaline region from pH 7 to pH 9
Metal ions	Iron and copper ions are poisonous for enzymes

Compatibility :

Nonionic surfactants	Generally very good
Anionic surfactants	Selective, some reduce the efficacy of the enzyme
Solvent based surfactants	Selective, some reduce the efficacy of the enzyme
Organic sequestrants	Generally good
Reducing / oxidizing agents	Reduce the efficacy of enzyme

Application Methods :
Exhaust application (Jigger, Soft-flow m/c, Winch, Wash-wheel)

Americos Amylase 180	: 1 - 2 g/l
Americos Anti-Crease Pro-76 A	: 0.5 - 1 g/l
pH	: 6 - 7
Time	: 30 - 60 min.

The actual concentration of Americos Amylase 180 depends on material to liquor ratio, agitation produced in m/c, cracking potential of sizing ingredients and equipment being used Drain the above liquor; give one hot wash and one cold wash. Alkaline washing with soda ash improves the removal efficiency of degraded sizing material.

2) Continuous Application (Jigger, Soft-flow m/c, Winch, Wash-wheel)

Americos Amylase 180	0.5 - 1% (owg)
pH	5.5 - 6.5
Impregnate the fabric at bath temperature between 70 - 80°C for 80 - 100% pick-up. Then steam it for 3 - 5min at 100°C In case of high temperature steaming, treat it for 1 - 2 min at 110C - 130°C.

Storage & Handling Precautions :

• Store in a cool place
• Avoid storing in direct sunlight
• The shelf life of Americos Amylase 180 is one year if stored below 40°C
• The usual precautions in keeping chemicals away from eyes and skin should be observed

Technical Service
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.

Introduction :
Americos Amylase 345 is a desizing agent based on bacterial alpha amylase. It is a mixture of highly concentrated alpha-amylase, wetting agent and lubricant to convert most of the sizes viz. starch, starch derivatives, synthetic sizes and their blends into water soluble products. Specifically it can be used for the following sizing materials and their combinations:

Advantages:

• Starch
• Starch / Polyvinyl alcohol
• Starch / Polyvinyl alcohol / Carboxyl Methyl cellulase (CMC)
• Starch / Polyvinyl alcohol / Acrylates

Americos Amylase 345 has three different types of actions. The built-in wetting agent ensures proper penetration of enzyme in the textile material. The enzyme then converts the starch sizes in to soluble dextrin products, which can be easily removed in the subsequent washings. Moreover, the in-built lubricant reduces fiber to fiber and fiber to metal friction. This unique combination prevents the formation of unwanted creases, streaks, lines and crack marks during desizing process.

Physical Property

Appearance	Viscous liquid
Chemical character	Blend of amylase enzyme and non-ionic surfactants
Ionic nature	Non-ionic
pH (on 1% solution)	5.5 – 6.5
Application pH	6 - 7
Application temperature	30 - 60°C

Stability

Hard water	Good, Ca2+ and Mg2+ ions increase the efficacy of the enzyme
Acids	Good from pH 5 to 7
Alkalis	Stable in alkaline region from pH 7 to 9
Metal ions	Iron and copper ions are poisonous for enzymes

Compatibility

Non-ionic surfactant	Generally very good
Anionic surfactant	Selective, some reduce the efficacy of the enzyme
Solvent based surfactant	Selective, some reduce the efficacy of the enzyme
Organic sequestrates	Generally good
Reducing / oxidizing agent	Reduce the efficacy of enzyme

Application Methods
Americos Amylase 345 can be applied by any one of the following techniques.

a) Exhaust application (jigger, soft-flow m/c and winch)

Americos Amylase 345	1 - 2 ml/l
Temperature	30-60°C
pH	6 -7
Time	30 - 60 min

After the treatment, drain the liquor; give one hot wash and then one cold wash. Alkaline washing with soda ash improves the removal efficiency of degraded sizing material.

b) Exhaust application (wash-wheel)

Americos Amylase 345	1% (owg)
Temperature	30-60°C
pH	6 -7
Time	30 - 60 min

First, raise the temperature of bath to 30 - 60oC. The machine should run for a minute to ensure proper mixing of chemicals. Adjust pH 6 - 7.

Thereafter, garment should be loaded. The desizing time can be varied from 30 - 60 min depending upon the type of fabric and chemical dosage.

3) Semi-continuous application (pad-batch process)

Americos Amylase 345	3 - 5 ml/l
pH	6 -7

Impregnate the fabric at 30-60°C for a pick-up of 80 - 100%. Store the fabric for 8 - 12 h; give one hot wash with soda ash, one plain hot wash and one cold rinse.

Effect of pH on Enzyme Activity

pH profile of Americos Amylase 345


Temperature profile of Americos Amylase 345

Storage & Handling Precautions

• Store in a cool place
• Avoid storing in direct sunlight
• The shelf life of Americos Amylase 345 is one year if stored below 40°C
• The usual precaution for chemicals should be observed

Technical Service
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.

Introduction :
Americos Amylase 380 enzyme is a concentrated thermostable bacterial amylase which should be used as a formulating base for desizing fabrics, sized with starch, starch derivatives, or blends of starch and other synthetic sizing materials. The strong amylolytic power of Americos Amylase 380 enzyme - especially at high temperatures - allows rapid conversion of the starch size into dextrins, which are easily removed by rinsing following impregnation with enzyme.

Americos Amylase 380 enzyme has long been the industry standard for quality, consistency, and stability. The formulation of the enzyme was designed to accommodate further addition of a variety of chemical auxiliaries in order to assist our customers in differentiating their desizing product line.

Americos uses state-of-the-art biotechnology production methods and this product contains enzyme (s) produced by a genetically modified organism.

Advantages:

• Concentrated for easy formulation into differentiated textile desizing products
• Active at high temperature
• Tolerates live steam

Typical Characteristics:

Activity	65000 TTAU/g (minimum)
Appearance	Brown liquid
Specific gravity	1.1
pH	6.0-8.0

The activity of Americos Amylase 380 enzyme is determined against an internal standard using the synthetic starch substrate blocked p-Nitrophenylmaltoheptoside (BGNP).

The resulting enzyme activity is expressed in Themostable Textile Amylase Units (TTAU)

Effect of temperature on the relative activity of Americos Amylase 380 enzyme



Effect of pH on the relative activity of Americos Amylase 380 enzyme (Substrate: 2% soluble starch held at 80°C)



Stability of Americos Amylase 380 enzyme over different temperature (pH 7)

Process Parameter	Operational Range
pH	5.0-9.0
Temperature	40°-110°C

Process Conditions:
Chelation:
Chelating agents should not be used with Americos Amylase 380 enzyme. Water hardness and common salt increase the stability of this enzyme.

Chemical auxiliaries:
Wetting agents, detergents, scouring agent, or solvent scours can be used with all applications of Americos Amylase 380 enzyme to enhance penetration and size swelling, along with assisting in the removal of waxes, soils, and synthetic sizing materials.

Typically, non-ionic surfactants are suitable for combination with enzymes whereas anionic and cationic surfactants may disrupt enzyme activity.

Lubricants are generally recommended to be used in combination with amylase enzymes during desizing to reduce the formation of crease marks and streaks. The addition of lubricants is especially recommended when desizing in rotary washers, in jet dye machines, or in preparation for denim washing.

Whenever auxiliary chemicals are used in conjunction with desizing enzymes, check to ensure that the auxiliaries are compatible with the enzyme.

Storage & Handling Precautions:

• Store in a cool place, preferably below 25°C
• Avoid storing in direct sunlight
• Prolonged storage above 30°C should be avoided
• The usual precaution for chemicals should be observed

Technical Service:
Information covering specific application of this product is available. Americos Industries will work with customers to enhance processes and solve problems. Let us know what you need and we will assist you.